rabbit anti-lysozyme polyclonal antibody  (Cell Marque)

Journal: eLife

Article Title: Nicotine enhances the stemness and tumorigenicity in intestinal stem cells via Hippo-YAP/TAZ and Notch signal pathway

doi: 10.7554/eLife.95267

Figure Lengend Snippet:

Article Snippet: Antibody , rabbit polyclonal anti-Lysozyme , Thermo Fisher Schientific , #PA5-16668 , IHC (1:50).

Techniques:

Journal: Journal of Veterinary Science

Article Title: Evaluation of porcine intestinal organoids as an in vitro model for mammalian orthoreovirus 3 infection

doi: 10.4142/jvs.23017

Figure Lengend Snippet: List of primary and secondary antibodies used in this study

Article Snippet: , Rabbit anti-Lysozyme polyclonal antibody , 1:200 , Cell Marque , 278A-15.

Techniques: Produced

Journal: BMC Infectious Diseases

Article Title: Lysozyme M deficiency leads to an increased susceptibility to Streptococcus pneumoniae -induced otitis media

doi: 10.1186/1471-2334-8-134

Figure Lengend Snippet: Immunolabeling showing the phenotypic expression of lysozyme in the E-tube . The schematic illustration showing right tympanic bulla and E-tube of a mouse (A). ME: middle ear, ET: Eustachian tube, NP: nasopharynx, TM: tympanic membrane, PO: pharyngeal orifice, TO: tympanic orifice, C: tubal cartilage, TL: E-tube lumen. Cross-sections at level b, c and d of both wild type and lysozyme M -/- mice are shown in B, C and D. Lysozyme is labeled in both the submucosal gland (SG) and the E-tube epithelium (arrow heads) of wild type mice while it is labeled predominantly in the submucosal gland (SG) of lysozyme M -/- mice. This indicates that lysozyme P of lysozyme M -/- mice is predominantly expressed in the submucosal gland, not in the tubal epithelium, considering that the polyclonal antibody recognizes both lysozyme M and lysozyme P. The secretion in the tubal lumen (arrow) is also immunolabeled with lysozyme in wild type mice, but not in lysozyme M -/- mice. All sections were immunolabeled with anti-human lysozyme antibody (1:1,000) and counterstained with hematoxylin. For each pair of images the left-hand panel displays sections from wild type mice and the right-hand displays sections from lysozyme M -/- mice. TC: tubal cartilage, TL: E-tube lumen. SG: submucosal gland. Original magnification: × 100.

Article Snippet: The membranes were incubated with a 1:5,000 dilution of rabbit polyclonal anti-human lysozyme antibody (EC 3.2.1.17) (DAKO) overnight at 4°C.

Techniques: Immunolabeling, Expressing, Labeling

Journal: BMC Infectious Diseases

Article Title: Lysozyme M deficiency leads to an increased susceptibility to Streptococcus pneumoniae -induced otitis media

doi: 10.1186/1471-2334-8-134

Figure Lengend Snippet: Compensatory up-regulation of lysozyme P in lysozyme M -/- mice . Conventional RT-PCR shows compensatory up-regulation of lysozyme P when substituting the lysozyme M gene with EGFP in lysozyme M -/- mice (A). Real time quantitative RT-PCR analysis shows that lysozyme P is remarkably up-regulated in the E-tube of lysozyme M -/- mice, compared to the lung (B). ND: not detected. Results were expressed as a fold-expression of mRNA quantity, taking the value of lysozyme P mRNA of the lung as 1. Values are given as mean ± standard deviation. n = 3. *: p < 0.05. The western blot (C) and its densitometry (D) show the expression of lysozyme protein in the E-tube homogenate. As expected, lysozyme M protein (arrow) is depleted and lysozyme P protein (arrow head) is up-regulated in the E-tube of lysozyme M -/- mice, compared to wild type mice. The soluble protein fraction of the E-tube homogenate was separated using 12.5% AU-PAGE electrophoresis, subsequently transferred onto PVDF membranes and labeled with a polyclonal anti-lysozyme antibody (1:5000). Signal was detected by exposure to X-ray film and quantified by the Quantity One ® software.

Article Snippet: The membranes were incubated with a 1:5,000 dilution of rabbit polyclonal anti-human lysozyme antibody (EC 3.2.1.17) (DAKO) overnight at 4°C.

Techniques: Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Standard Deviation, Western Blot, Electrophoresis, Labeling, Software